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Use of Hydrocyclones for Mammalian Cell Retention ...

Cited by: 25

The Potential of Hydrocyclone Application for

DOI: 10.6000/1927-3037.2013.02.04.2 Corpus ID: 84350518. The Potential of Hydrocyclone Application for Mammalian Cell Separation in Perfusion Cultivation Bioreactors @article{Elsayed2013ThePO, title={The Potential of Hydrocyclone Application for Mammalian Cell Separation in Perfusion Cultivation Bioreactors}, author={Elsayed A Elsayed and Mohamad A. Wadaan}, journal={International Journal of ...

Separation of CHO cells using hydrocyclones

Cited by: 58

Use of Hydrocyclones for Mammalian Cell Retention ...

A hydrocyclone with a volume of 2.56 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow ...

The Potential of Hydrocyclone Application for

DOI: 10.6000/1927-3037.2013.02.04.2 Corpus ID: 84350518. The Potential of Hydrocyclone Application for Mammalian Cell Separation in Perfusion Cultivation Bioreactors @article{Elsayed2013ThePO, title={The Potential of Hydrocyclone Application for Mammalian Cell Separation in Perfusion Cultivation Bioreactors}, author={Elsayed A Elsayed and Mohamad A. Wadaan}, journal={International Journal of ...

The Use of Hydrocyclones for Mammalian Cell

They separated HeLa cells with a separation efficiency of 81% using a 10-mm Dorr-Oliver hydrocyclone working at 4 bar. The cell viability was insignificantly affected due to the short residence ...

Application of Hydrocyclone for Cell Separation in ...

2016-10-18  Application of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Cultures Vom Fachbereich für Biowissenschaften und Psychologie der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung des Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) genehmigte Dissertation von Elsayed Ahmed Elsayed Ahmed

The Use of Hydrocyclones for Mammalian Cell

A new type of hydrocyclone was designed and used in continuous mammalian cell cultivation processes. The apparatus gave cell separation efficiencies as high as 99%. Due to the fact that a 5-L-bioreactor was used, a sub-optimal configuration of overflow and underflow diameters was used in order to obtain high cell viabilities combined with ...

Application of Hydrocyclone for Cell Separation in ...

Cited by: 4

Use of Hydrocyclones for Mammalian Cell ... - DeepDyve

A hydrocyclone with a volume of 2.56 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow corresponding to pressure drops of 0.4 to 1.3 bar) the hydrocyclone was characterized with regard to flow split (underflow‐to‐overflow ratio) and flow ratio (underflow to supply). Cultures of BHK and HeLa ...

Use of Hydrocyclones for Mammalian Cell ... - DeepDyve

A hydrocyclone with a volume of 2.56 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow corresponding to pressure drops of 0.4 to 1.3 bar) the hydrocyclone was characterized with regard to flow split (underflow‐to‐overflow ratio) and flow ratio (underflow to supply). Cultures of BHK and HeLa ...

Application of Hydrocyclone for Cell Separation in ...

2020-4-22  In the present work, the application of a hydrocyclone for cell separation in continuously perfused cultures of mammalian cells was investigated. The characterization of the hydrocyclone performance showed, that a pressure range of 0.85 to 1.40 bar is most suitable to achieve a reliable and stable separation process. Secondly, the effect of hydrocyclone operation on the cell viability and the ...

PDF Kostenlos Application of Hydrocyclone for Cell ...

hydrocyclone mammalian cells - palestra-oefentherapie.nl A hydrocyclone with a volume of 2.56 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow ...

Acoustic Settler, Centrifuge, Hydrocyclone - Animal

2020-5-20  Hydrocyclone. A detailed description of the hydrocyclone for mammalian cell culture can be found in Castilho's review (Castilho and Medronho 2002). Hydrocyclones are often used to separate solids from liquids or liquids with different densities in chemical processes.

The Application of Dielectric Spectroscopy and ...

2015-5-7  the cells with all the essential nutrients and removing all the waste by-products. The cells are usually retained within the reactor by a cell separating device such as a spin filte r, cross flow filter, hydrocyclone or acoustic cell settler, Figure 1A. However, these cell

Hydrocyclones as cell retention device for CHO perfusion ...

2020-4-8  In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary cells. The effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an auxiliary pump to control the perfusate flow rate, and tubing size in the recirculation loop were evaluated in batch ...

Application of Hydrocyclone for Cell Separation in ...

Application of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Cultures (Tienda española) Elsayed Ahmed Elsayed Ahmed (Autor) Previo. Indice, Datei (88 KB) Lectura de prueba, Datei (160 KB) ISBN-10 (Impresion) 3865376347: ISBN-13 (Impresion) 9783865376343: ISBN-13 (E-Book) 9783736916340: Idioma: Deutsch: Numero de paginas: 154:

Application of Hydrocyclone for Cell Separation in ...

The present work was carried out to investigate the possibility of hydrocyclone application for cell separation in mammalian cell perfusion cultures. Firstly,...

Application of Hydrocyclone for Cell Separation in ...

Bücher bei Weltbild.de: Jetzt Application of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Cultures von Elsayed Ahmed Elsayed Ahmed versandkostenfrei bestellen bei Weltbild.de, Ihrem Bücher-Spezialisten!

Application of Hydrocyclone for Cell Separation in ...

Bücher bei Weltbild.de: Jetzt Application of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Cultures von Elsayed Ahmed Elsayed Ahmed versandkostenfrei bestellen bei Weltbild.de, Ihrem Bücher-Spezialisten!

Application of Hydrocyclone for Cell Separation in ...

The present work was carried out to investigate the possibility of hydrocyclone application for cell separation in mammalian cell perfusion cultures. Um Ihnen ein besseres Nutzererlebnis zu bieten, verwenden wir Cookies.

hydrocyclone cell harvesting

hydrocyclone. Most hydrocyclone manufacturers are basically producing the same designs that they have been using for the past 50 years. In contrast the GIW Hydrocyclone is the result of a fresh original look at mining processing needs and modern technology. GIW's straightforward tie bolt and clamp design is a breeze to install and maintain. Get ...

Application of hydrocyclones for continuous

Hydrocyclone can be successfully used for cell separation in continuous mammalian perfusion bioreactors. Cells adapt themselves to the shear forces inside the HC without being adversely affected by the pressure. Higher separation efficiencies up to 96%

Elsayed Ahmed, E: Application of Hydrocyclone for

The second part of the work aimed to study the effect of different pressures generated by the hydrocyclone on the cell viability and the separation efficiency using two cell lines, BHK and HeLa cells. The separation efficiency increased by increasing the pressure.

Application of hydrocyclones for continuous

Effect of hydrocyclone operating pressure on separation efficiency and separated cell quality. Results (Figure 2) showed that increasing operating pressure from 0.85 to 1.30 bar increased the average total separation efficiency from 89 to 95%.Additionally, results also showed that hydrocyclone preferably separates more viable cells in the underflow, and hence, back to the bioreactor system.

Z__eLearning_MediaAsset_Fermentation_Broch_Complete ...

2018-7-30  Z__eLearning_MediaAsset_Fermentation_Broch_Complete_Upstream_Solutions_SBI1511-e_生物学_自然科学_专业资料 2人阅读次下载 Z__eLearning_MediaAsset ...

Application of hydrocyclone for cell separation in ...

Application of hydrocyclone for cell separation in mammalian cell perfusion cultures [Elektronische Ressource] / von Elsayed Ahmed Elsayed Ahmed : Application of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Cultures Vom Fachbereich für Biowissenschaften und Psychologie der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung des Grades eines

MEETING ABSTRACT Open Access Application of

2017-8-25  - Hydrocyclone can be successfully used for cell separa-tion in continuous mammalian perfusion bioreactors. - Cells adapt themselves to the shear forces inside the HC without being adversely affected by the pressure. - Higher separation efficiencies up to 96% can be

Data Hydrocyclone SBL2001-e - Literaturwissenschaft

hydrocyclone mammalian cell retention device for single-use and reusable bioreactors perfusion technologies overflow (harvest) feed feed product specification. Anmelden Registrieren; Verstecken. Data Hydrocyclone SBL2001-e. Universität. Universität Potsdam. Kurs. Literaturwissenschaft 1 FRZVL1. Hochgeladen von.

Method for separating viable cells from cell

2005-4-12  1. Method of separating viable mammalian or insect cells from cell suspensions, wherein the cell suspension is introduced, by way of an inlet device, tangentially into the cylinder space of at least one hydrocyclone and is allowed to flow in a downwardly directed, spirally circling course through the cone of the hydrocyclone, a suspension of increased cell concentration being collected at the ...

Application of hydrocyclones for continuous

Application of hydrocyclones for continuous cultivation of SP-2/0 cells in perfusion bioreactors: Effect of hydrocyclone operating pressure BMC Proceedings , Dec 2011 Elsayed A Elsayed , Roland Wagner

Application of hydrocyclone for removal of yeasts

Application of hydrocyclone for removal of yeasts from alcohol fermentations broth. ... Recent applications of hydrocyclones for separation of microbial cells and mammalian cells are promising by cutting down the dimensions sizes , . The findings may be useful in applying the concepts for separation of proteins and large biomolecules in future.

Method for separating viable cells from cell

2003-9-4  Method for separating viable cells from cell suspensions . United States Patent Application 20030166269 . Kind Code: A1 . Abstract: The invention relates to a method of separating viable cells from cell suspensions using hydrocyclones, which method can, especially, be carried out continuously. ...

Download [PDF] Mammalian Cell Culture Free Online

Large-Scale Mammalian Cell Culture is composed of papers presented as part of a symposium sponsored by the American Chemical Society Division of Microbial and Biochemical Technology at the 188th American Chemical Society National Meeting, held at Philadelphia, Pa., on Aug. 27, 1984.

Separation of CHO cells using hydrocyclones. -

The best performance was obtained with a 10-mm Dorr-Oliver hydrocyclone, resulting in a separation efficiency of 81%. These experimental values agree with computational fluid dynamics (CFD) predictions that high levels of separation efficiencies for mammalian cells could be achieved with small-diameter hydrocyclones (Medronho et al. 2005).

Separation of CHO cells using hydrocyclones ...

Separation of CHO cells using hydrocyclones Separation of CHO cells using hydrocyclones Pinto, Rodrigo; Medronho, Ricardo; Castilho, Leda 2007-11-14 00:00:00 Cytotechnology (2008) 56:57–67 DOI 10.1007/s10616-007-9108-x OR IGINAL RESEARCH Rodrigo C. V. Pinto Æ Ricardo A. Medronho Æ Leda R. Castilho Received: 3 April 2007 / Accepted: 20 October 2007 / Published online: 14

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